Gene region | Primer name | Reference | Primer sequence (5′ ➔ 3′) | PCR Protocol |
---|---|---|---|---|
ITS (ITS1, 5.8S gene, and ITS2) | p17F | [57] | 5′-CTACCGATTGAATGGTCCGGTGAA-3′ | 94 °C 5 min; 28 cycles of 94 °C 1 min, 48 °C 1 min, 72 °C 1 min; 72 °C 7 min; 10 °C paused |
26S-82R | 5′-TCCCGGTTCGCTCGCCGTTACTA-3′ | |||
5 | [58] | 5′-GGAAGTAAAAGTCGTAACAAGG-3′ | 94 °C 5 min; 30 cycles of 94 °C 1 min, 55 °C 1 min, 72 °C 1 min, 45 s; 72 °C 10 min; 10 °C paused | |
4 | 5′-TCCTCCGCTTATTGATATGC-3′ | |||
matK | 3F_kimF | [59] | 5′-CGTACAGTACTTTTGTGTTTACGAG-3′ | 98 °C 45 s; 35 cycles of 98 °C 10 s, 52 °C 30 s, 72 °C 40 s; 72 °C 10 min; 10 °C paused |
IR_kimR | 5′-ACCCAGTCCATCTGGAAATCTTGGTTC-3′ | |||
psbA-trnH | psbA_F | [60] | 5′-GTTATGCATGAACGTAATGCTC-3′ | 95 °C 4 min; 35 cycles of 94 °C 30 s, 55 °C 1 min, 72 °C 1 min; 72 °C 10 min; 10 °C paused |
trnH_R | 5′-CGCGCATGGTGGATTCACAATCC-3′ | |||
trnL-F | c | [61] | 5′-CGAAATCGGTAGACGCTACG-3′ | 94 °C 3 min; 30 cycles of 93 °C 1 min; 55 °C 1 min, 72 °C 2 min; 10 °C paused |
f | 5′-ATTTGAACTGGTGACACGAG-3′ |